Plant Hormone extraction, analysis, and quantitation * Hormone extraction and identification has been made possible due to advances in chemical analysis, chromatographic techniques, and mass spectrometry. * It is difficult to extract and identify plant hormone from plant extract as the extract contains major metabolites, such as sucrose, also hormones concentrations in plant tissues are low up to more than ten times compared to other material in plant tissue. * Another thing that effects identification and extraction is the complexity in structure of plant hormones. * Chemical properties of phytohormone also effect hormone analysis, quantification and extraction. * Wide range of chemical properties, many hormones (e.g., gibberellic acid and brassinosteroids) are quite hydrophobic, while hormones like auxins and cytokinins are more hydrophilic. * Keeping the diversity of solubility, Structure and chemical nature in view, tissue homogenates are subjected to “clean up” or enrichment met...
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