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Extraction, analysis and quantification of plant hormones

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Cryopreservation

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Cryopreservation is a conservation method that allows the long-term storage of samples at low temperature up-to -196C making sure that it does not affects cell's viability. It is an important and efficient method for preservation of world's genetic resources. It allows long term conservation of biological material Its simplicity and applicability make it an efficient method. The plant material stored can be- Seed, Shoot tip, Zygotic embryo, somatic embryo, Pollens, different types of plant cells, tissue and organs. Various means for storage at low temperature: At -79°C over solid CO2 At-80 °C in Deep Freezers  At -150 °C in Vapor phase At -196 °C in Liquid Nitrogen These varying temperatures are also called as Sub-Zero temperature. PRINCIPLE The Sub-Zero temperature (below0 °C) makes the cells suspended due to slow down of biological reactions including metabolism, cellular transport, division etc. The cells remain in this suspended state until the temperature is restored. This
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Economic Botany of Datura plant

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