Skill Of Questioning : English : Lesson plan : Micro Lesson

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Extraction, analysis and quantification of plant hormones

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Plant Hormone extraction, analysis, and quantitation * Hormone extraction and identification has been made possible due to advances in chemical analysis, chromatographic techniques, and mass spectrometry. * It is difficult to extract and identify plant hormone from plant extract as the extract contains major metabolites, such as sucrose, also hormones concentrations in plant tissues are low up to more than ten times compared to other material in plant tissue. * Another thing that effects identification and extraction is the complexity in structure of plant hormones. * Chemical properties of phytohormone also effect hormone analysis, quantification and extraction. * Wide range of chemical properties, many hormones (e.g., gibberellic acid and brassinosteroids) are quite hydrophobic, while hormones like auxins and cytokinins are more hydrophilic. * Keeping the diversity of solubility, Structure and chemical nature in view, tissue homogenates are subjected to “clean up” or enrichment met
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Cryopreservation

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Cryopreservation is a conservation method that allows the long-term storage of samples at low temperature up-to -196C making sure that it does not affects cell's viability. It is an important and efficient method for preservation of world's genetic resources. It allows long term conservation of biological material Its simplicity and applicability make it an efficient method. The plant material stored can be- Seed, Shoot tip, Zygotic embryo, somatic embryo, Pollens, different types of plant cells, tissue and organs. Various means for storage at low temperature: At -79°C over solid CO2 At-80 °C in Deep Freezers  At -150 °C in Vapor phase At -196 °C in Liquid Nitrogen These varying temperatures are also called as Sub-Zero temperature. PRINCIPLE The Sub-Zero temperature (below0 °C) makes the cells suspended due to slow down of biological reactions including metabolism, cellular transport, division etc. The cells remain in this suspended state until the temperature is restored. This
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Economic Botany of Datura plant

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Economic Botany- Morphology, Cultivation and Uses of D atura stramonium  Botanical Name: Datura stramonium Family: Solanaceae Part used: Leaves, flowers, roots and seeds. The genus name Datura is derived from dhatura, the Bengali name for the plant. Datura species, also called thornapple, devil’s apple, angel’s trumpet (in a broad sense) or devil’s trumpet, have their main center of origin in Mexico and the southwest United States (US). It is an aggressive invasive weed in temperate climates and tropical climates across the world. Botanical Description : Dhatura is an annual plant and the length of Dhatura plant, tall and has a pungent odor thus Datura stramonium is a bushy, smooth, annual. The branching stem is spreading and leafy Root: cylindrical, brown, rough-splintered, with lateral branches. Stem: Cylindrical, dichotomously branched, blackish-purple to dark purple in colour, with a very short internode. Leaf: alternately arranged, with a pointed border. Leaves a
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